IGEM:IMPERIAL/2007/Notebook/2007-9-19

From OpenWetWare

Jump to: navigation, search
August
SMTWTFS
1234
567891011
12131415161718
19202122232425
262728293031
September
SMTWTFS
1
2345678
9101112131415
16171819202122
23242526272829
30
October
SMTWTFS
123456
78910111213
14151617181920
21222324252627
28293031


Purification of LuxR-His

  • Today we innoculated 2 litres of LB medium with E.coli containing the luxR-his plasmid (pMU117) to be grown to an O.D.600 0.5. After this the cells were harvested and frozen overnight.
  • In addition an SDS-PAGE gel was run from a small sample of the soluble proeins from these cells, that had been purified using an affinity column (Nickel column for His tag).
  • The gel was left to destain overnight
Personal tools