IGEM:IMPERIAL/2007/Notebook/2007-9-20

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August
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1234
567891011
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262728293031
September
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1
2345678
9101112131415
16171819202122
23242526272829
30
October
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123456
78910111213
14151617181920
21222324252627
28293031


Purification of LuxR-His

  • The SDS-PAGE gel of the soluble protein fraction was checked in the morning. The gel showed bands at 28kDa, the size of LuxR-His, in the soluble fraction and the insolble fraction. In addition, from the soluble fraction that was run through the column it can be seen that there is also this band present in some of the fractions. From these results we decided to try to purifiy a large amount of the LuxR-His from the soluble fraction of 1 litre cells.
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