IGEM:IMPERIAL/2007/Notebook/2007-9-28

From OpenWetWare

Jump to: navigation, search
August
SMTWTFS
1234
567891011
12131415161718
19202122232425
262728293031
September
SMTWTFS
1
2345678
9101112131415
16171819202122
23242526272829
30
October
SMTWTFS
123456
78910111213
14151617181920
21222324252627
28293031


Optimum DNA concentration

  • Aim today was to get the optimum DNA concentration for the pTet-GFP and the pTet-lixR-pLux-GFP construct. The protocols can be found Here
  • The constructs were tested for 0, 1, 2, 4 and 6ug of DNA.
  • The fluorescence over a period of 6 hours was recorded every hour for all the concentrations.
  • It was found that at 25°C pTet-lixR-pLux-GFP behaves as expected, i.e. the optimum output is for a mass between 2 and 4ug of DNA. The results can be foud here
  • For pTet-GFP construct, at 37°C, the fluorecence was highest at the end of the day for 6ug of DNA.
  • The optimum DNA concentration that was tested is between 2-6µg of DNA, however the difference between 2µg,4µg,6µg is negligible. The results can be foung here
Personal tools