IGEM:IMPERIAL/2007/Notebook/2007-9-6

From OpenWetWare

Jump to: navigation, search
August
SMTWTFS
1234
567891011
12131415161718
19202122232425
262728293031
September
SMTWTFS
1
2345678
9101112131415
16171819202122
23242526272829
30
October
SMTWTFS
123456
78910111213
14151617181920
21222324252627
28293031


Testing of Homemade S30 Cell Extract

  1. Set up 4 reactions with the following composition
    • S30 Cell extract 16.2μl
    • Reaction buffer 30μl
    • Pyruvate kinase 3.1μl
    • rNTPs 1μl
    • DNA/ddH20 4μl
    • ddH20 5.7μl
  2. Left at 37°C incubator
    • 1. Cell extract reaction with pTet-GFP
    • 2. Cell extract reaction without pTet-GFP (negative control)
    • 3. Cell extract reaction with pT7-GFP
    • 4. Cell extract reaction without pT7-GFP (negative control)
Personal tools