IGEM:IMPERIAL/2007/Notebook/2007-9-7

From OpenWetWare

Jump to: navigation, search
August
SMTWTFS
1234
567891011
12131415161718
19202122232425
262728293031
September
SMTWTFS
1
2345678
9101112131415
16171819202122
23242526272829
30
October
SMTWTFS
123456
78910111213
14151617181920
21222324252627
28293031


LuxR Purification

SDS-PAGE done. Columns: Gel Marker, soluble (diluted), soluble, insoluble (dilted), insoluble
SDS-PAGE done. Columns: Gel Marker, soluble (diluted), soluble, insoluble (dilted), insoluble

We did a preliminary test on two protocols that were used to purify LuxR. One involve the extraction of LuxR in soluble form, while the other was the extraction of LuxR in the form of inclusion bodies.

The results suggest that soluble form protocol appears to yield increased amounts of LuxR protein (29kDa). This corresponds near to the marker set at 31kDa (the 5th line on the marker).


Vesicles

Results

Sample 8, produced on 04.09.2007 was looked at again under the fluorescence microscope, and vesicles were still present at a density similar to that of the first observation.

Personal tools