IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation

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Cell by Date: Implementation


Contents


Chassis Focus

Chassis Phase 1 Phase 2 Phase 3
E.coli X
In-Vitro X X X
In-Veso X X X


Test Constructs

Type of Promoter Test Constructs In vivo In Vitro In veso Status
Constitutive T7 Promoter
PT7 promoting GFP
Available Available
  • Stock Availability: Midiprepped (Tested)
  • Submission to Registry: Available
Constitutive E.coli Promoter
Ptet</sub promoting GFP
Available Available
  • Stock Availability: Midiprepped (Tested)
  • Submission to Registry: Available
Inducible E.coli Promoter
PBad promoting GFP
Cancelled Cancelled Cancelled
  • Stock Availability: Cancelled
  • Submission to Registry: N/A
Constitutive E.coli Promoter
PcI promoting GFP
N/A N/A N/A
  • Stock Availability: Not Working
  • Submission to Registry: N/A
Inducible E.coli Promoter
pBad promoting GFP and pcI promoting araC
Cancelled Cancelled Cancelled
  • Stock Availability: Cancelled
  • Submission to Registry: N/A


Ligation Table

More details on building the constructs.

Lab Notebook

  • Week 5 (6 Aug): Building Constructs
  • Week 6 (13 Aug): Building Constructs / Phase 1
  • Week 7 (20 Aug): Phase 1 / Phase 2
  • Week 8 (27 Aug):
  • Week 9 (3 Sep):
  • Week 10 (10 Sep):


August
SMTWTFS
1234
567891011
12131415161718
19202122232425
262728293031
September
SMTWTFS
1
2345678
9101112131415
16171819202122
23242526272829
30
October
SMTWTFS
123456
78910111213
14151617181920
21222324252627
28293031

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