IGEM:IMPERIAL/2007/Projects/Hrp System/Devices

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Hrp Characterisation: Devices


Contents


Characterisation


This is a prioritised list of devices utilising the Hrp system that we would like to characterise.

Device 1: One input


This device is a pHrpL promoter utilising a single RS operon only.
A single promoter activates HrpR and HrpS expression simultaneously.

Device 2: Two inputs

AND with one inversion: pHrpL promoter - RS and V
AND with one inversion: pHrpL promoter - RS and V



This device simulates a basic AND gate with one inverted input.
Again, HrpR and HrpS are controlled by a single promoter. HrpV expression is
dependent on a different promoter. The R and S proteins activate the pHrpL
promoter while the V protein represses it.

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