IGEM:IMPERIAL/2007/Projects/Hrp System/Testing/Protocol Hrp
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Equipment
- Twinkle Plate Reader Fluorometer
- 96 well plate
- Eppendorf Tubes
- Gilson Pippettes
- Plastic Curvetts
- Incubator 37o shaker
- Vortex and Centrifuge
- Spectrometer
Materials and Reagents
- M9 minimal media 50μg/ml Ampicillin
- Inducer of promoter at varying concentrations
- Recombinant acGFP of known concentration
- Agar Plates
- E.coli strain containing vector
Protocols
Promoter Test Construct
The promoter pLux is to be tested at 8 varying ranges of inducer concentration.
- pLux will have a range of 0 to 100μM AHL added at intervals of 12.5μM Protocol
Device Test Construct
For this test construct we initially need to run a preliminary test in order to find out the time intervals and input ranges we need. We will start the preliminary test using the range of concentrations used initially for promoters.