Vitro v Vivo
|Quick and Simple||Laborious and Time Consuming|
|Good Control||Poor Control|
|Circular and Linear DNA||Circular DNA Only|
|Minimal Protein Degradation||Endogenous Proteases|
|Can synthesise toxic proteins||Toxic proteins kill the cells|
|Short lifespan||Long lifespan|
|Less characterized, not so popular||Well characterized, popular|
Yeah, but In Vitro has its own problems.
|Less characterized, not so popular||Well, that's what we are trying to change.|
|Expensive||Can't do much about this...|
|Short lifespan||Golly gee, what can we do about that?|
But how is this Synthetic Biology?
True Biological Engineering
Built from the Bottom Up
- Vesicles can make Synthetic Biology more useful in the Real WorldTM
Right then, tell me about them vesicles.
As we saw last week, we can make vesicles.
But what about Characterisation?
The process is not well characterised.
There is no modelling.
There is no data.
Characterisation takes a long time.
|Dear Santa, this year I would like five crates of elbow grease...|
...and what about the applications?
Vesicles aren't necessary for Infector Detector.
Cell By Date needs a long life span, not served by In Vitro systems.
Time, once again...
The application experiments in-veso are out of reach.