IGEM:IMPERIAL/2008/Prototype/Wetlab/Sandbox

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Home The Project B.subtilis Chassis Wet Lab Dry Lab Notebook



To do list

Promoter and RBS Characterisation

  • Define the characterisation
  • Inducible promoters - find literature to help us define the inducer concentration we will test for our transfer function.
  • Inducible promoters - Order the inducers
  • Light inducible promoters - Find the equipment and the range of lights required
  • write protocol
  • work out how to use plate reader

Biomaterial Characterisation

  • How are we going to measure biomaterial production - simply via gel or via microscopy

Motility

  • How to culture the B.subtilis for motility assays

Characterisation

Inducible

Constitutive

Light Sensitive


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