IGEM:IMPERIAL/2008/Prototype/Wetlab/Shopping List
From OpenWetWare
< IGEM:IMPERIAL | 2008 | Prototype | Wetlab
| Home | The Project | B.subtilis Chassis | Wet Lab | Dry Lab | Notebook |
|---|
| Materials | Quantity | Company | Order No. | Cost | Date |
| Glycerol | |||||
| Glucose | |||||
| MgSO4.7H2O | 500g | Sigma | M1880 | ||
| Casamino Acids | |||||
| Yeast Extract | |||||
| CaCl2 | 500g | Sigma | C1016 | ||
| (NH4)2SO4 | 500g | Sigma | A4418 | ||
| KH2PO4 | |||||
| Sodium Citrate | |||||
| EGTA | 10g | Sigma | E3889 | ||
| EDTA | |||||
| Trypton | |||||
| Minimal Salts | 250g | Sigma | M6030 | ||
| NaCl | 2.5kg | Fisher | BP358 | ||
| Ammonium Citrate | 100g | Sigma | F5879 | ||
| Casein hydrolysate | |||||
| PEG 6000 | 250g | Sigma | 81255 | ||
| KCL | 1kg | Fisher | BP366 | ||
| MgCl | 100g | Sigma | M8266 | ||
| Midi kit | |||||
| Mini Kit | |||||
| Gel Extraction | |||||
| Ampicilin | 25g | Sigma | A9518 | ||
| Kanamycin | 25g | Sigma | K4000 | ||
| Tetracyclin | 5g | Sigma | T7660 | ||
| PCR clean up kit | |||||
| Pfu ultra fusion | |||||
| PBS x 50 | |||||
| Agar | |||||
| Agarose | |||||
| Polyacrylamide | |||||
| Tris | 1kg | Fisher | BP152 | ||
| Ammonium persulfate (APS) | 25g | Fisher | BP179 | ||
| SDS | 1 litre 20% | Fisger | BP1311 | ||
| TEMED | 25ml | Fisher Scientific | T/p190/04 | ||
| TAEx50 | |||||
| Protein Marker | |||||
| DNA Marker | |||||
| Ethanol | |||||
| Acetic Acid | |||||
| Commasie Brilliant Blue R | |||||
| β Mercapto Ethanol | |||||
| DTT | |||||
| Bromophenol Blue | |||||
| Grams Iodine Solution | 8FOZ | Sigma | HT902 | ||
| IPTG | |||||
| HEPES | 1kg | Fisher | BP310 | ||
| Glycine | 1kg | Fisher | BP318 |
