These assay are ordered in step-wise order based on what testings should be done first
Secondary carbon source selection for CRP promoter
- Choose the secondary carbon sources most suitable for CRP promoter activity
- Constitutive promoter activity will be tested using methods originally described by Jason Kelly. This assay should be used to provide the data required for the calculation of a PoPs output from the CRP promoter (for a given set of conditions).
Determining best concentration of IPTG
- Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell
Determining concentration of glucose and time delay
- Relate the time it takes to reach switch point for varying initial concentrations of glucose.