- M9 Minimal Media:
Measure out the following reagents and dissolve them in 1000ml of sterile H20: Disodium Phosphate = 6.0g
Potassium dihydrogen phosphate = 3.0g
Sodium Chloride = 0.5g
Ammonium Chloride = 1.0g
Autoclave the M9 stock solution.
Resuspend a frozen cell pellet in 1ml of M9 minimal media.
1) Pipette inoculum into M9 minimal media supplimented with glucose.
2) Immediatly take OD and electronic glucose readout.
3) Place in incubator (shaking, 37 degrees centigrade).
4) Repeat OD and glucose measurements every 30 minutes.