IGEM:IMPERIAL/2009/Specifications

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Specifications

Tuesday 21st July 2009

Module 1

  • Produce an enzyme (protein)
  • How much? How long for? (GFP as a reporter gene?)
  • Be able to control/regulate
  • Cell proliferation?
  • Inducible
  • Do not secrete out of cell
  • Switch off once desired compound is produced


Module 2

  • Induce encapsulation
  • Adequate mechanical/chemical deposition (bacterial control)
  • Acid resistance (has to bypass stomach - capsule composition)
  • Release in small intestine (module 5 delivery)
  • Non toxic
  • Be able to control & regulate; thickness, uniformity
  • Time taken for encapsulation to take place (want ‘fast’ encapsulation)


Module 3

  • 100% killing (high as possible killing rate)
  • What is the probability of survival? (how do we know it has worked?)
  • Failsafe mechanism (use timer?) vs double killing mechanism
  • Do not want proteases to degrade our product
  • Destruction of cell membrane? (to release our product)
  • Non-toxic, fast, reliable
  • when do we want to induce killing? Run in parallel? Overlap? Timer?
  • how to induce? Light? Timer? Can’t be chemical


Timer

  • Accurate
  • pre-set (decide time to preset)
  • module 1 → module 2
  • module 2 → module 3

-delay timer for fail safe?

  • module 5??


Compound

Ranking 1-3; most important(1) to least important (3).

  • Sequence available 1
  • Expertise/equipment available in College 1
  • Stable - know production vs. degradation rates 2
  • Compatible with available chassis 1
  • Genes/pathway known 1
  • Enzyme - specificity? Single enzyme, no crosstalk 1
  • Functions in gut temp./environment. Survives and is absorbed 1
  • No existing cheap solution → want advantage / benefit 1
  • Optimum level/ dose control → does it naturally oscillate with the day? 1
  • Impact, appeal, wow factor, high profile 2
  • Not covered by previous iGEM teams 3


Provisional Flowchart

Currently under construction:


Flowchart

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