IGEM:IMPERIAL/Protocols/western blot
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- Grow overnight in a 5 mil culture
- Make a fresh day culture 400ul of overnight culture + 4.6 ml of fresh medium (LB)
- Grow fresh culture for 1.5hrs
- Remove 1ml This will be your controll
- Add IPTG to the remaining 4ml Final conc should be 0.5-1 milimolar So add 4ul of 1 molar IPTG
- Grow for 2-3 hrs
- Centrafuge down
- Go to Keran's Lab (on the same floor as kirstin)
- Add Lysis Buffers
- Run on gel
You should get this