IGEM:IMPERIAL/2006/Results/ABS490 Calibration

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Aim

The aim of this experiment was to make a calibration curve to be able to convert Abs490, which is measured by the plate reader, into OD600.

Method

  • A dilution series of different OD600s was created using the in-lab spectrophotometer and overnight culture of cells.
  • These dilutions were also assessed via the spectrophotometer to make sure they were near their targetted OD.
  • 3 x 200μL of each dilution was pipetted into a 96 well plate and then Abs490 was read by the Victor3 plate-reader
  • The cells and the dilutions were kept on ice to prevent any increase in OD

Results

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