IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/09

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  • Miniprep of 6 samples:
  • I13602 -- 1
  • I13602 -- 2
  • J23119 + E0420 -- 1
  • J23119 + E0420 -- 2
  • J23119 + i13507 -- 1
  • J23119 + i13507 -- 2
  • Inoculation of 6 samples.
  • E0430
  • E0430
  • I13504
  • I13504
  • I13522
  • I13522
  • Restriction of plasmids obtained from minipreps (11 +6)
  • Gel loading (Intact plasmid + Restricted plasmids for each samples)
  • Gel order:
  • Row 1: I13602(1A2)(plasmid); I13602(1A2)(insert); I13602(1A2)(plasmid); I13602(1A2)(insert); S03879(1C3)(Plasmid); S03879(1C3)(Insert); S03879(1C3)(Plasmid); S03879(1C3)(Insert); LADDER; J23119+E0420(1A2)(Plasmid);J23119+E0420(1A2)(Insert);J23119+E0420(1A2)(Plasmid);J23119+E0420(1A2)(Insert); K117005(1C3)(plasmid);K117005(1C3)(Insert).
  • Row 2: K117005(1C3)(plasmid);K117005(1C3)(Insert); J23119+I13507(1A2)(Plasmid); J23119+I13507(1A2)(Insert); J23119+I13507(1A2)(Plasmid); J23119+I13507(1A2)(Insert); J13002(1C3)(plasmid)(2); J13002(1C3)(insert)(2); J13002(1C3)(plasmid)(1);J13002(1C3)(insert)(1); J13002(1C3)(plasmid)(1);J13002(1C3)(insert)(1); B0014(1C3)(plasmid); B0014(1C3)(insert);B0014(1C3)(plasmid); B0014(1C3)(insert); J23119(1C3)(Plasmid); J23119(1C3)(Insert); J23119(1C3)(Plasmid); J23119(1C3)(Insert).
  • HAPPY BIRTHDAY BAWLA :)


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