Entry title
- OD v/s Conc expt: (DH5aplha; K12Z1; DH5aplha(P2 Plasmid); lacI- cells(P2 plasmid))
- 3ml inoculum prepared.
- 1.5ml added to Flasks
- OD and conc were measured every 30 mins.
- OD was calculated after resspending the cell pellet in Saline.
- AHL confirmation:
- AHL experiment: (DH5alpha; P2 in LacI- cells; T9005 cells)
- Incubate all 3 cell types.
- 2 mixtures made:
- 1) 2ml T9005+ 2ml LacI- + 2 ml 2xyt broth -----> with Amp
- 2) 2ml T9005+ 2ml DH5aplha + 2 ml 2xyt broth --> without Amp
- GFP expt:
- inoculate DH5alpha cells in 5ml culture.
- Add IPTG 3 hrs after incubation
- Measure CFP after 5 hrs
- The following was done as controls for restriction; ligation; Alkaline phosphatase treatment; and Transformation.
- Restricted J23119(1A3) with S/P.
- Restricted K156011(PCR) with X/P.
- Alkaline Phosphatase (AP) of half of J23119(S/P) and half of K156011(X/P)
- Following ligations performed: (AP = alkaline phosphatase treated)
- C1) J23119(AP) + K156011;
- C2) J23119(AP) + K156011(AP);
- C3) J23119 + K156011(AP);
- C4) J23119 + K156011;
- C5) J23119(AP)
- C6) J23119
- Transformations of:
- C1 through C6; C7(unligated J23119(S/P)); C8(unligated J23119(AP)(S/P))
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