IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/10

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  • Miniprepped the 6 samples which were inoculated yesterday viz. E0430(2); I13504(2); I13522(2)
  • Digested all 6 plasmids with E/P.
  • Gel was run.
  • gel order:
  • BLANK; I13522(1A2)(1); I13522(E/P)(1); I13522(1A2)(2); I13522(E/P)(2); I13504(1A2)(1);I13504(E/P)(1); LADDER; I13504(1A2)(1);I13504(E/P)(1); E0430(1A2)(1); E0430(E/P)(1);E0430(1A2)(1); E0430(E/P)(1);LADDER.
  • G set of ligations were done:
  • J13002(2) + E0020 ---- 3A (Chl)
  • J13002(2) + E0420 ---- 3A (Chl)
  • J13002(1) + E0020 ---- 3A (Chl)
  • J13002(1) + E0020 ---- 3A (Chl)
  • J23119 + I13504 ---- 3A (Chl)
  • K145151 + E0420 ---- 3A (Chl)
  • K145151 + E0430 ---- 3A (Chl)
  • K145151 + I13504 --- 3A (Chl)
  • S03879 + I13507 ---- 3A (Chl)
  • J23119 + E0430 ----- 3A (Chl)



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