IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/09/30

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  • Order: S+I(1,2); S+I(3,4)
  • Gel run with entire template:
  • NO PCR product found
  • PCR of S+I7 with primers 1 and 2
  • PCR program:
  • 1) 98°C for 2min;
  • 2) 98°C for 30s;
  • 3) 64.4°C for 30s;
  • 4) 72°C for 45s;
  • go to 2 for 30 times
  • 5) 72°C for 30min;
  • 6) 4°C hold
  • inoculated DH5alpha in SOB for competant cell preparation.



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