IGEM:MIT/2005/Completed Work

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scFv.TT (J07012-14.B0015)
   Image:Check.gif digest B0015 E/X, recipient
   Image:Check.gif obtain digested scFvs E/S, prefix donor
   Image:Check.gif gel purify B0015
   Image:Check.gif ligate J07012-14.B0015
   Image:Check.gif transform
   Image:Check.gif overnights for miniprep
   Image:Check.gif identify correct clones (digest), streak plates
   Image:Check.gif overnights for freezer stock
   Image:Check.gif freezer stocks scFv.B0015
   
scFvs: 4M5.3, S101A, 4420
   Image:Check.gif biobrick ends
   Image:Check.gif digest scFv + pSB1AK3-1 plasmid w/ E/S 
   Image:Check.gif gel purify each
   Image:Check.gif ligate together
   Image:Check.gif transform
   Image:Check.gif overnights for miniprep
   Image:Check.gif identify correct clones (digest)
   Image:Check.gif overnights for freezer stock
   Image:Check.gif freezer stocks
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