IGEM:MIT/2005/Friday 7/29 Meeting Agendas
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Input
- chemically competent
- cells with oligos fluresced, cells with fluorescein unknown
- competent vs. regular
- what next?
- lets do this again
- FACS this afternoon
- Will and Max are going to meet with an advisor
Head Receiver
- scFvs are biobricks
- attach terminator to 3' end
- will attach promoter::rbs
- getting materials to test
ToxR
- MalE and PhoA are on their way
- Lets talk about what we're doing next week
- New option: have them built locally
FecA
- Whats done:
- FecA wt, FecI, FecR
- To do:
- quick change for FecA, FecR, PhoA
- FecA promoter error: need new promoters
- scFv linker: SG original linker not the best, according to some. Our linker should be fine. =o)
- Still obtaining Fur- strain -- hopefully soon
Signal Processing
- hoping our parts will come in
- ordered assembled parts
Actuator
- ready and waiting