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Transformation of J45400

  • ligated J45399 (4 of them...1A, 1B, 1C, and 2A) to R0011, in A/T backbone, to make J45400 in A/T backbone. We still need to check the sequencing of the J45399s to see which ones were right...hopefully one of the 4 I used was correct. there wasn't a lot of R0011, so I chose 1A, 1B, and 1C b/c they looked the best on the gel, and 2A for the heck of it.

Osmy+E0840 for plate reader

  • transformed osmy+E0840 FN for the plate reader...used an amp plate b/c I wasn't sure what the resistance was.
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