IGEM:MIT/2007/Notebook/2007-9-25

From OpenWetWare

Jump to: navigation, search

Double Digests

8 µl J04630
0.5 µl BSA
5µl NEB3 Buffer
1µl XbaI
1µl PstI
34.5µl H20
---------------
50µl Total
9 µl J04650
0.5 µl BSA
5µl EcoRI Buffer
1µl XbaI
1µl PstI
33.5µl H20
---------------
50µl Total
5 µl Q04510
0.5 µl BSA
5µl NEB3 Buffer
1µl EcoRI
1µl SpeI
37.5µl H20
---------------
50µl Total
9 µl E0840
0.5 µl BSA
5µl NEB3 Buffer
1µl XbaI
1µl PstI
33.5µl H20
---------------
50µl Total
6 µl E1010
0.5 µl BSA
5µl NEB3 Buffer
1µl XbaI
1µl PstI
36.5µl H20
---------------
50µl Total
5 µl J04630
0.5 µl BSA
5µl NEB2 Buffer
1µl EcoRI
1µl SpeI
37.5µl H20
---------------
50µl Total
  • 37C @ 2:15pm

Gel Electrophoresis

Top of Gel (Lanes to the left)

Lane 1 = (2µl 1 kb ladder) + (4µl loading buffer) + (14µl H2O)
Lane 3 = (10µl dd E1010) + (4µl loading buffer) + (6µl H2O)
Lane 5 = (24µl purified Mer PCR (BH) ) + (6µl loading buffer) : 30µl Total

Botton of Gel

Personal tools