IGEM:Melbourne/2008/Notebook/BioClock/2008/08/05

From OpenWetWare

Jump to: navigation, search
Melbourne iGEM 2008 -- BioClock Main project page
Previous entry      

Done today:

  • Red light retest.

4 transformations:

  • Cph8 + pPL-PCB
  • Cph8
  • PCB
  • -ve control


Protocol:

  • 2μL DNA
  • RU1012
  • 30 min ice
  • 1 min 42°C
  • 1 hour at 37°C + LB

Each transformation plated onto 2 plates, one of which is wrapped in foil. Placed in 37°C room at genetics which light on

Plates: Amp Kan Chlor Arabinose (2mM) IPTG (1mM) X-gal

Alex


Personal tools