IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/28

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Date: 08/28/13 People in lab: Emily Puleo

Title: Prepare norCB and nosZ primers

Start Time: 11:50 AM

Purpose: To increase the concentration of primer

Protocol: Dr. Westenberg's own protocol

Products:

Sample Label Description
8/28/13 norCB F 0.58 norCB forward primer, .58 uM concentration
8/28/13 norCB R 0.52 norCB reverse primer, .52 uM concentration
8/28/13 nosZ F 0.62 nosZ forward primer, 0.62 uM concentration
8/28/13 nosZ R 0.64 nosZ reverse primer, 0.64 uM concentration

Notes: See 7/31 notes for example calculations

Stop Time:

Next: PCR of norCB and nosZ

Date: 08/28/13 People in lab: Hannah Frye

Title: PCR of norCB and nosZ for TOPO cloning

Start Time: 1:00 PM

Purpose: Add iGEM prefix&suffix to the genes, then preparing samples for TOPO-TA cloning

Protocol: LTM ed. 2 Exceptions: 1. Annealing temp: 65C 2. Elongation time: 7 min 3. Final primer conc. <0.1uM

Products:

Sample Label Description
8/28/13 norCB A norCB plus prefix&suffix
8/28/13 nosZ A nosZ plus prefix&suffix

Stop Time: 7:45 PM


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