IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/30

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Date: 08/30/13 People in lab: Kelsey Crossen

Title: Gel electrophoresis of 8/28 PCR products

Start Time: 9:00 AM

Purpose: Check if PCR was successful

Protocol: iGEM LTM ed. 2

Well 1 2 3 4 5 6 7 8
Sample Ladder norCB A 8/28 nosZ A 8/28

Results: No product visible

Stop Time:

Next: Adjust PCR and try again

Date: 08/30/13 People in lab: David Pohlman

Title: Making competent cell seed plates

Purpose: To grow fresh DH5alpha cells for making competent cells

Protocol: iGEM LTM ed. 2 - Streaking from frozen stock

Products: 2 Seed plates - DH5alpha 8/30/13

Next: Inoculate into LB broth


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