IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/28

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Date: 09/28/13 People in lab: Levi Palmer

Title: Digest backbone, ligate into circular plasmid

Start Time: 1:16 PM

Purpose: To circularize the linear backbones

Protocol: LTM ed. 2


Sample Label Description
D LP 9/28 Digested ampicillin backbone with E and P
L1 LP 9/28 Circular ampicillin backbone

Notes: IMPORTANT: Don't do this, it won't work. Must digest with S and X, not E and P! -LP 11/21/13

Stop Time: 3:30 PM

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