IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/30

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Date: 09/30/13 People in lab: Levi Palmer

Title: Transform backbone into competent cells

Start Time: 4:12 PM

Purpose: To test for competence of cells and amplify amp backbone

Protocol: LTM ed. 2

Products:

Sample Label Description Source Label Quantity
9/30 Amp Backbone CT E. coli transformed with Amp backbone L LP 9/28 2
9/30 pUC19 BB CT E. coli transformed with pUC19 control plasmid pUC 19 DNA 2

Results: 10/01: Transformations did not work.

Notes: Performed with both amp and pUC 19 plasmids

Stop Time: 7:00 PM

Next: Check for success, if succesful, inoculate amp cells into LB broth.



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