IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/25

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Date: 11/25/13 People in lab: Blythe Ferriere

Title: PCR norV for PstI digest and gel electrophoresis/extraction, then digest with PstI

Start Time: 12:15 PM

Purpose: To perform the steps necessary for site-directed PCR mutagenesis

Protocol: LTM ed. 2

Products:

Sample Label Description Source Label Quantity
11/25/13 norV A1 norV gene with prefix and suffix E. coli 7/31 2
11/25/13 norV A3 Control to make sure PCR from a PCR works, norV from norV 11/25/13 norV A1 2
11/25/13 norV A5 PCR of norV with shorter annealing time E. coli 7/31 2
11/25/13 GE 1 Extraction of A5 11/25/13 norV A5 2
11/25/13 norV A7 PCR of extracted PCR products 11/25/13 GE1 2
11/25/13 D1 Digestion of A7 with PstI 11/25/13 norV A7 2
Well 1 2 3 4 5 6 7 8
Sample Ladder A1 Ladder A2
Well 1 2 3 4 5 6 7 8
Sample Ladder A5 Ladder A6
Well 1 2 3 4 5 6 7 8
Sample Ladder A7 Ladder A8

Results:

Notes: Used norCB program, used water to elute DNA from gel purification

Stop Time:

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