IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/02/27

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Date: 02/27/14 People in lab: Levi Palmer

Title: Blue white colony screening of hmp

Start Time: 11:00 AM

Purpose: To confirm colonies have hmp gene

Protocol: 1. Add 15 uL of 1000x X-gal to LB Amp plates 2. Plate 0.2 uL overnight culture 3. Add 15 uL SOC to plate to help spread cells 4. Spread with beads 5. Incubate overnight 37C

Products:

Sample Label Description Source Label Quantity
2/27 Poss hmp Xgal LP Transformed cells on amp+xgal Poss hmp LP 2/26 1

Results: 2/28/14: Lawn growth, unusable for B/W colony screening

Notes:

Stop Time: 12:00 PM

Next: Inoculate any white colonies in LB Amp broth

Date: 02/27/14 People in lab: Levi Palmer

Title: norCB primer dilution

Start Time: 12:00 PM

Purpose: To prepare new primers for PCR, bring down to ~0.7 uM

Protocol: 1. To make 1 ug/uL stock, add 430 uL milliQ to 0.43 mg F primer, and 400uL to .4 mg R primer, 2. To make (1X) working solution, add 1uL stock to 99uL milliQ for final concentration of 10 ng/uL or ~0.7 uM

Products:

Sample Label Description Source Label Quantity
2/27 F norCB primer 0.7 uM F primer norCB IDT norCB F 2/24 1
2/27 R norCB primer 0.7 uM R primer norCB IDT norCB R 2/24 1

Notes: Stored in -20C freezer in norCB box

Stop Time: 1:00 PM

Next: Inoculate any white colonies in LB Amp broth


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