IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/03/19

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Date: 03/19/14 People in lab: Levi Palmer, Caleb Trecazzi

Title: PCR of hmp and TOPO-TA cloning with transformation

Start Time: 4:00 PM

Purpose: To amplify hmp gene from E. coli and put into TOPO vector to transform into E. coli(DH5alpha)

Protocol: LTM ed. 2, Invitrogen TOPO-TA cloning manual Exceptions: 1. 6min incubation time

Products:

Sample Label Description Source Label Quantity
hmp TOPO 3/19 1 hmp in TOPO vector, plated 1uL PCR product used E.coli 8/6/13 4
hmp TOPO 3/19 2 hmp in TOPO vector, plated 2.5uL PCR product used E.coli 8/6/13 4
hmp TOPO 3/19 3 hmp in TOPO vector, plated 4uL PCR product used E.coli 8/6/13 4

Results:

Notes: Variable amounts of PCR product used to see what works best.

Stop Time: 7:00 PM

Next: Blue/white colony screening to check for colonies with insert



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