IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/06/06

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Date: 6/6/14 People in lab: Caleb Treccazi

Title: Digest of chloramphenical resistant vector Start Time: 7 pm Purpose: To have the correct backbone ready for parts when done Protocol: LTM Ed. 2 Digests Exceptions: Products:

Sample Label Description Source Label Quantity
Chlor Vector 6/6 Chlor resistant backbone cut at EcoRI and pstI iGEM Distribution Kit 2012 1





Results: Notes: Stop Time: 9 pm Next: Ligation of norV A1 and A2 with chlor vector 6/6

Date: 6/6/14 People in lab: Caleb Treccazi

Title: Ligation of norV into chlor resistant backbone Start Time: 9 pm Purpose: To have a funcitonal part Protocol: LTM Ed. 2 Ligation Exceptions: Products:

Sample Label Description Source Label Quantity
L 1 6/6 norV gene in chlor vector A 1 12/2 and chlor vector 6/6 1
L 2 6/6 norV gene in chlor vector A2 12/2 and chlor vector 6/6 1




Results: Notes: Stop Time: 9:15 pm Next: Chemical transformations



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