IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/10/04

Cyanobacteria Mediated Filtration of Coal Flue Gas

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Date: 10/4/14 People in lab: Kelsey Crossen Title: Minipreps Start Time: 2:50 pm Purpose: Isolate igem Plasmid cocntaining hmp and a promoter+RBS site Protocol: LTM Ed. 2 Miniprep Exceptions: Products: Sample Label Description Source Label Quantity MP1 10/4 ng/uL = 210.9; 260/280 = 2; 260/230 = 2.1 BC5 10/3 1 MP2 10/4 ng/uL = 144.7; 260/280 = 2.12; 260/230 = 2.23 BC6 10/3 1 MP3 10/4 ng/uL = 446.8; 260/280 = 1.9; 260/230 = 1.61 BC7 10/3 1 MP4 10/4 ng/uL = 173.4; 260/280 = 2; 260/230 = 2.03 BC8 10/3 1 MP5 10/4 ng/uL = 144.8; 260/280 = 1.97; 260/230 = 1.68 BC9 10/3 1 MP6 10/4 ng/uL = 234.5; 260/280 = 2.02; 260/230 = 2.17 BC10 10/3 1 MP7 10/4 ng/uL = 314.3; 260/280 = 1.9; 260/230 = 2.21 BC11 10/3 1 MP8 10/4 ng/uL = 195.9; 260/280 = 1.92; 260/230 = 2.19 BC12 10/3 1 Results: Notes: Stop Time: 4:55 pm Next: Digest with P and X and run gel to check for insert and vector Date: 10/4/14 People in lab: Kira Buckowing Title: Innoculation from CT1-2 10/2 Start Time: 3:15 pm Purpose: To get more liquid cultures of hmp in psB1C3 Protocol: LTM Ed. 2 Innoculation Exceptions: Products: Sample Label Description Source Label Quantity BC1 10/4 hmp CT1 10/2 1 BC2 10/4 hmp CT1 10/2 1 BC3 10/4 hmp CT2 10/2 1 BC4 10/4 hmp CT2 10/2 1 Results: Notes: Stop Time: 3:30 pm Next: Glycerol stocks of one and minipreps of the other 3