IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/30/Zhangs

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Double digestion of CI
System for front insert:

10μLplasmid
2μL10×H beffer
1.5μLEcoRI
1.5μLSpeI
5μLddH2O
20μLTotal

4 h digested
Electrophoresis
Result (from left to right)
CI, CI plasmid control, marker

Gel: 0.072gPN volume: 216μL
Gel extraction for CI insert

Ligation Insert:CI Vector:1-23L, 1-4H
System
(1)7:1 system

7μLCI insert
1μLvector
1μL10× Ligase buffer
1μLLigase
10μLTotal

(2)3:1 system

3μLCI insert
1μLvector
1μL10× Ligase buffer
1μLLigase
4μLddH2O
10μLTotal

Control

0CI insert
1μLvector
1μL10× Ligase buffer
1μLLigase
7μLddH2O
10μLTotal

6 samples in total, 2 for 1-23L, 2 for 1-4H, 2 for 1-23L & 1-4H controls

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