IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/27/Zhenglin Yang

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11:00 Double digestion of purified T7 polymerase PCR production

12:30 Tube cultivation of T3 promoter + GFP

14:30 Tube cultivation of T3 promoter parts

19:30 Tube cultivation of T3 Polymerase

21:00 Purify the digestion production of T7 polymerase

22:30 Ligation of T7 polymerase and 1-2M vector

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