IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/14/Zhangs
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Electrophoresis and Gel Extraction for T7ptag+1-23L insert
Result (from left to right)
T7ptag①, T7ptag②, Marker
Gel: T7ptag+1-23L 0.125gPN volume: 375μL
Ligate the T7ptag+1-23L insert and 9 RBS vector
System
| 3μL | insert |
| 1μL | RBS vector |
| 1μL | 10× Ligase buffer |
| 1μL | Ligase |
| 4μL | ddH2O |
| 10μL | Total |
