IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/23/Zhangs
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Double digestion for Terminator vector
Digestion system:
30μL | Terminator plasmid |
5μL | 10×M beffer |
1μL | EcoRI |
1μL | XbaI |
13μL | ddH2O |
50μL | Total |
After 90min digestion, add: 1~2μL CIAP+5μL CIAP buffer
Ligate the CI insert and Terminator vector.
Transforming the CI insert + Terminator vector ligation product.