IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/07/14/Zhangs

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Ligate the 1-5J RBS+CI+1-23L insert and T7 promoter vector
System

3μLinsert
1μLT7p vector
1μL10× Ligase buffer
1μLLigase
4μLddH2O
10μLTotal
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