IGEM:PKU Beijing/2009/Notebook/AND Gate 2/2009/07/19/Zhenglin Yang

Received the Three Counter Plasmid

13:00 Transformation:
Transform the three counters plasmid into the DH5α cell and apply the bacteria template to K+ plate

15:00 Plate cultivation
37℃ plate culture

16:00 Double digestion for GFP vector

23:00 Tube cultivation
Pick single clones on the plate and 37℃ tube culture in shaker