IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/19/Shan Shen
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10:30 Miniprep 2-4O, 1-18P, low-copy bi-stable system Concentration:
| Number of the plasmids | Concentration(ng/μL) |
| 2-4O | 350 |
| 1-18P | 250 |
| Low-copy bi-stable system | 125 |
12:00 Digest 1-18P, 2-4O, low-copy plasmids. 1-18P by Spe1 and Pst1; 2-4O by Xba1 and Pst1; low-copy bi-stable system by EcoR1 and Pst1.
18:00 Recycle those digestion products.
22:40 Ligation of high-copy bi-stable system fragments and high-copy backbone.
| Total | 10μL |
| Vectors | 1μL |
| Inserts | 7μL |
| T4 ligase | 1μL |
| Buffer | 1μL |
