IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/06/Shuke Wu

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Plasmid mini prep
6 RBS;
lacI;
araC;

Double digest
6 RBS: Spe1 1uL, Pst1 1uL, plasmid 4uL, Buffer 2uL, water 12uL
lacI, araC: Xba1 1uL, Pst1 1uL, plasmid 4uL, Buffer 2uL, water 12uL
37 ℃ 4 hour

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