IGEM:Paris Bettencourt 2012/Protocols/Medium

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Medium

NB : Don't forget to write on the bottle, 'igem, date, which medium (eg. LB), initials'

LB, aka Luria Bertani

Use LB powder from BD (Difco LB Broth, Miller)

Concentration : 25g/L

  • In a Beaker
    • 50g into 2L of dH2O
    • or 25 g into 1L, and so on.
  • Solubilize by stirring
  • Poor in a bottle, which is twice the volume of what you add into it (eg. 500mL of LB into a bottle of 1L)
  • Autoclave, 121°C, 20' (prog. 4)
  • have fun

LBA, aka Luria Bertani Agar

Use LB powder from BD (Difco LB Agar, Miller)

NB : cannot be solubilize at room temperature, it will be solubilize after autoclaving

Concentration : 40g/L

  • In a 1L Bottle : 20g LBA + 500mL dH2O
  • Autoclave, 121°C, 20' (prog. 4)
  • have fun
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