IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/04

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Léa Miniprep of 10mL cultures elution in 35µL warm water Ps/weak RBS/LuxR Ps/med RBS/LuxR Ps/stand RBS/LuxR Pm/med RBS/LuxR Pm/weak RBS/LuxR Pm/stand RBS/LuxR RBS/LuxI Ps Pm Term Nanodrop of minipreps Sample name 260/280 260/230 ng/µL Ps/weak RBS/LuxR 1.68 0.76 74.9 Ps/med RBS/LuxR 1.45 0.55 127.3 Ps/stand RBS/LuxR 1.73 0.93 62.8 Pm/med RBS/LuxR 1.68 0.91 134.5 Pm/weak RBS/LuxR 1.47 0.63 132.1 Pm/stand RBS/LuxR 1.85 1.60 118.9 RBS/LuxI 1.71 0.95 134.4 Ps 1.88 1.37 91.2 Pm 1.79 1.54 100.4 Term 1.88 1.65 85.6 Raphaël Midiprep of 100mL culture attC-t-attC-mRFP Nanodrop of midiprep Sample name 260/280 260/230 ng/µL attC-t-attC-mRFP 1.89 2.27 370.2 Aleksandra and Raphaël Overnight cultures in 10ml attC/term/attC/mRFP #10, 11 cultures - Kan term - Amp RBS/LuxI/term in Turbo cells plate 1, 2 colonies - Amp RBS/LuxI/term in Turbo cells plate 2, 2 colonies - Amp RBS/LuxI/term in TOP10, 3 colonies - Amp