IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/13

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Aleksandra
Glycerols

  • stRBS/LacZα/LuxI


Minipreps, elution in 35µl warm water

  • Ps/sRBS/LuxR/attC/t/attC/mRFP col4
  • Pm/sRBS/LuxR/attC/t/attC/mRFP col1
  • Ps/mRBS/LuxR/attC/t/attC/mRFP col4
  • Ps/wRBS/LuxR/attC/t/attC/mRFP col4
  • mRBS/TetA(C)/LuxI col1
  • wRBS/TetA(C)/LuxI col2
  • sRBS/TetA(C)/LuxI col2
  • wRBS/LacZα
  • sRBS/LacZα
  • LuxI
  • pLux/GFP
  • sRBS/LacZα/LuxI (same as glycerol)


Restriction digest with SpeI and PstI : 20µl miniprep + 2x1µl enzyme + 2.5µl B2 + 0.25µl BSA

  • Ps/sRBS/LuxR/attC/t/attC/mRFP col4
  • Pm/sRBS/LuxR/attC/t/attC/mRFP col1
  • Ps/mRBS/LuxR/attC/t/attC/mRFP col4
  • Ps/wRBS/LuxR/attC/t/attC/mRFP col4
  • wRBS/LacZα
  • sRBS/LacZα
  • pLux/GFP
  • Pm/wRBS/LuxR/attC/t/attC/mRFP


Restriction digest with XbaI and PstI : 20µl miniprep + 2x1µl enzyme + 2.5µl B2 + 0.25µl BSA

  • sRBS/LacZα/LuxI
  • mRBS/TetA(C)/LuxI col1
  • wRBS/TetA(C)/LuxI col2
  • sRBS/TetA(C)/LuxI col2
  • LuxI (30µl miniprep + 2x1µl enzyme + 3.7µl B2 + 0.37µl BSA)


Gel electrophoresis
Gel 1

  • Ps/sRBS/LuxR/attC/t/attC/mRFP col4
  • -
  • Pm/sRBS/LuxR/attC/t/attC/mRFP col1
  • -
  • Ps/mRBS/LuxR/attC/t/attC/mRFP col4
  • -
  • Ps/wRBS/LuxR/attC/t/attC/mRFP col4
  • -
  • mRBS/TetA(C)/LuxI col1
  • -
  • wRBS/TetA(C)/LuxI col2
  • Ladder 1kb

Image:Gel1308 1.jpg


Gel 2

  • sRBS/TetA(C)/LuxI col2
  • Ladder 100bp
  • LuxI
  • LuxI
  • Ladder 1kb
  • sRBS/LacZα/LuxI

Image:Gel1308 2.jpg


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