IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/17

From OpenWetWare

Jump to: navigation, search
Memo cell Main project page
Previous entry      Next entry
Population counter Main project page
Previous entry      Next entry

Aleksandra
Transformations of TURBO cells with 5µl ligation product, plating with 150µl

  • 1)Pm/sRBS/LuxR + wRBS/TetA(C)/LuxI
  • 2)Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI
  • 3)Ps/mRBS/LuxR + wRBS/TetA(C)/LuxI
  • 4)Ps/sRBS/LuxR + wRBS/TetA(C)/LuxI
  • 5)Pm/sRBS/LuxR + mRBS/TetA(C)/LuxI
  • 6)Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI
  • 7)Ps/mRBS/LuxR + mRBS/TetA(C)/LuxI
  • 8)Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI
  • 9)Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI
  • 10)Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI
  • 11)Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI
  • 12)Ps/sRBS/LuxR + sRBS/TetA(C)/LuxI

Controls:

  • 13)Pm/sRBS/LuxR
  • 14)Ps/wRBS/LuxR
  • 15)Ps/mRBS/LuxR
  • 16)Ps/sRBS/LuxR
  • 17)pUC19 (positive control)


Léa
Glycerols and minipreps

  • wRBS/LacZα/LuxI, 2 cultures
  • sRBS/LacZα/LuxI, 2 cultures
  • LuxI


Nanodrop of the minipreps

Sample name 260/280 260/230 ng/µl
wRBS/LacZα/LuxI cult1 1.902.40 422.1
wRBS/LacZα/LuxI cult2 1.95 2.34362.3
sRBS/LacZα/LuxI cult1 1.92 2.37387.4
sRBS/LacZα/LuxI cult21.94 2.35 415.2
LuxI1.94 2.37 207.0


Restriction digest with XbaI and PstI : 20µl miniprep + 2x1µl enzyme + 2.5µl B3 + 0.25µl BSA

  • wRBS/LacZα/LuxI, 2 cultures
  • sRBS/LacZα/LuxI, 2 cultures
  • LuxI


Gel electrophoresis

  • Ladder 100bp
  • -
  • wRBS/LacZα/LuxI, cult1
  • -
  • wRBS/LacZα/LuxI, cult2
  • -
  • sRBS/LacZα/LuxI, cult1
  • -
  • sRBS/LacZα/LuxI, cult2
  • -
  • LuxI
  • Ladder 1kb


The restriction didn't work Image:Gel1708 1.jpg


Overnight cultures

  • wRBS/LacZα/LuxI, 4 colonies
  • sRBS/LacZα/LuxI, 4 colonies
  • LuxI, 1 culture from glycerol


Raphaël
Overnight cultures

  • pLux/LuxI
  • Pm/wRBS/LuxR/attC/t/attC/mRFP
  • Ps/sRBS/LuxR/attC/t/attC/mRFP, 4 colonies


Aleksandra
Overnight cultures

  • 2)Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, 4 colonies (1 didn't grow)
  • 6)Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, 4 colonies
  • 8)Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, 1 colony
  • 9)Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, 1 colony
  • 10)Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies
  • 11)Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies (2 didn't grow)




Personal tools