IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/20

Memo cell	Main project page Previous entry      Next entry
Raphaël Restriction digest with EcoRI-HF and SpeI : 20µl miniprep + 2x1µl enzyme + 2.5µl B4 + 0.25µl BSA Left arm HK022 Mut-Mut Left arm HK022 WT-Mut Left arm λ Mut-Mut Left arm λ WT-Mut Left arm WT-WT Restriction digest with EcoRI-HF and XbaI : 20µl miniprep + 2x1µl enzyme + 2.5µl B4 + 0.25µl BSA Kan resistance cassette Restriction digest with XbaI and PstI : 20µl miniprep + 2x1µl enzyme + 2.5µl B3 + 0.25µl BSA Right arm HK022 Mut-Mut Right arm HK022 WT-Mut Right arm λ Mut-Mut Right arm λ WT-Mut Restriction digest with SpeI and PstI : 20µl miniprep + 2x1µl enzyme + 2.5µl B2 + 0.25µl BSA LacZα expression cassette
Population counter	Main project page Previous entry      Next entry
Aleksandra Minipreps Ps/wRBS/LuxR + attC/term/attC/mRFP Ps/mRBS/LuxR + attC/term/attC/mRFP Ps/sRBS/LuxR + attC/term/attC/mRFP Pm/sRBS/LuxR + attC/term/attC/mRFP pLux wRBS/LacZ sRBS/LacZ wRBS/TetA(C) mRBS/TetA(C) 2x RBS/LuxI Raphaël Restriction digest with XbaI and PstI : 20µl miniprep + 2x1µl enzyme + 2.5µl B2 + 0.25µl BSA wRBS/TetA(C) mRBS/TetA(C) 2x RBS/LuxI Restriction digest with SpeI and PstI : 20µl miniprep + 2x1µl enzyme + 2.5µl B2 + 0.25µl BSA Ps/wRBS/LuxR + attC/term/attC/mRFP Ps/mRBS/LuxR + attC/term/attC/mRFP Ps/sRBS/LuxR + attC/term/attC/mRFP Pm/sRBS/LuxR + attC/term/attC/mRFP pLux wRBS/LacZ sRBS/LacZ Aleksandra and Raphaël Gel electrophoresis Ps/wRBS/LuxR + attC/term/attC/mRFP Ladder 1kb Ps/mRBS/LuxR + attC/term/attC/mRFP - Ps/sRBS/LuxR + attC/term/attC/mRFP - Pm/sRBS/LuxR + attC/term/attC/mRFP - pLux - wRBS/LacZ File:Gel2008 1.jpg Gel electrophoresis sRBS/LacZ - wRBS/TetA(C) - mRBS/TetA(C) - RBS/LuxI RBS/LuxI Ladder 1kb - - - -> The gel was unfortunately damaged... thus we have lost a lot of DNA and the migration was abnormal. File:Gel2008 2.jpg Léa Gel extraction Ps/sRBS/LuxR + attC/term/attC/mRFP Pm/sRBS/LuxR + attC/term/attC/mRFP pLux wRBS/LacZ