IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/23

Memo cell	Main project page Previous entry      Next entry
Population counter	Main project page Previous entry      Next entry
Raphaël Minipreps Ps/sRBS/LuxR/attC/t/attC/mRFP Ps/mRBS/LuxR/attC/t/attC/mRFP Ps/wRBS/LuxR/attC/t/attC/mRFP Restriction digest with SpeI and PstI : 20µl miniprep + 2.5µl B2 + 2x1µl enzymes + 0.25µl BSA Ps/sRBS/LuxR/attC/t/attC/mRFP Ps/mRBS/LuxR/attC/t/attC/mRFP Ps/wRBS/LuxR/attC/t/attC/mRFP Gel electrophoresis Ps/wRBS/LuxR/attC/t/attC/mRFP - Ps/mRBS/LuxR/attC/t/attC/mRFP - Ps/sRBS/LuxR/attC/t/attC/mRFP Ladder 1kb File:2308 1.jpg Gel extraction Ps/sRBS/LuxR/attC/t/attC/mRFP Ps/mRBS/LuxR/attC/t/attC/mRFP Aleksandra PCR, 1µl miniprep (13/08) + 2x1µl primers 0.025ng/µl + 10µl water + 13µl 2x quick load mix, Tm=60, Telong=68 wRBS/TetA(C) mRBS/TetA(C) sRBS/TetA(C) Gel electrophoresis wRBS/TetA(C) mRBS/TetA(C) sRBS/TetA(C) Ladder 1Kb File:2308 2.jpg -> bands of agarose in the fridge