IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/31

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Colony PCR
Gradient PCR (53-73°C to determine hybridization temperature of VF2+VR)
Primers : VF2 + VR

  • 12x PmS-WTet III
  • 12x PsM-MTet III


Minipreps

  • WLac-LuxI II
  • WLac-LuxI III
  • SLac-LuxI II
  • SLac-LuxI III
  • pLux-LuxI II
  • pLux-LuxI III
  • PsS-MTet II
  • PsS-MTet III
  • PmS-WTet II
  • PmS-WTet III
  • PsM-MTet II
  • PsM-MTet III
  • PsW-MTet II
  • PsW-MTet III
  • PsW-WTet II
  • PsW-WTet III
  • PsM-WTet II
  • PsM-WTet III
  • PsS-WTet II
  • PsS-WTet III


Restriction digest with SpeI and EcoRI-HF

  • pLux-LuxI II
  • pLux-LuxI III
  • PsS-MTet II
  • PsS-MTet III
  • PmS-WTet II
  • PmS-WTet III
  • PsM-MTet II
  • PsM-MTet III
  • PsW-MTet II
  • PsW-MTet III
  • PsW-WTet II
  • PsW-WTet III
  • PsM-WTet II
  • PsM-WTet III
  • PsS-WTet II
  • PsS-WTet III


Restriction digest with XbaI and PstI

  • WLac-LuxI II
  • WLac-LuxI III
  • SLac-LuxI II
  • SLac-LuxI III


Gel electrophoresis

  • pLux-LuxI II
  • Ladder 1Kb
  • pLux-LuxI III
  • -
  • PsS-MTet II
  • -
  • PsS-MTet III
  • -
  • PmS-WTet II
  • -
  • PmS-WTet III
  • -
  • PsM-MTet II
  • -
  • PsM-MTet III
  • -
  • PsW-MTet II


Gel electrophoresis

  • PsW-MTet III
  • -
  • PsW-WTet II
  • -
  • PsW-WTet III
  • -
  • PsM-WTet II
  • -
  • PsM-WTet III
  • -
  • PsS-WTet II
  • -
  • PsS-WTet III
  • Ladder 1Kb
  • -
  • WLac-LuxI II



Gel electrophoresis

  • Ladder 1Kb
  • WLac-LuxI III
  • -
  • SLac-LuxI II
  • -
  • SLac-LuxI III


Overnight cultures

  • Ps/wRBS/LuxR/attC/term/attC/mRFP
  • Ps/mRBS/LuxR/attC/term/attC/mRFP
  • Ps/sRBS/LuxR/attC/term/attC/mRFP
  • Pm/sRBS/LuxR/attC/term/attC/mRFP
  • wRBS/TetA(C)/LuxI
  • mRBS/TetA(C)/LuxI
  • sRBS/TetA(C)/LuxI
  • wRBS/LacZα
  • sRBS/LacZα
  • LuxI
  • J13507/pLux/GFP
  • pLux
  • attP (in CmR)


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