Raphaël
Minipreps
- Ps/wRBS/LuxR/attC/term/attC/mRFP
- Ps/mRBS/LuxR/attC/term/attC/mRFP
- Ps/sRBS/LuxR/attC/term/attC/mRFP
- Pm/sRBS/LuxR/attC/term/attC/mRFP
- wRBS/TetA(C)/LuxI
- mRBS/TetA(C)/LuxI
- sRBS/TetA(C)/LuxI
- wRBS/LacZα
- sRBS/LacZα
- LuxI
- I13507/pLux/GFP
- pLux
- attP (in CmR)
Restriction digest
15µl DNA + 2x1µl enzymes + 2µl buffer + 0.2µl BSA
With SpeI and PstI (B2):
- Ps/wRBS/LuxR/attC/term/attC/mRFP
- Ps/mRBS/LuxR/attC/term/attC/mRFP
- Ps/sRBS/LuxR/attC/term/attC/mRFP
- Pm/sRBS/LuxR/attC/term/attC/mRFP
- wRBS/LacZα
- sRBS/LacZα
- pLux
With XbaI ansd PstI (B3):
- wRBS/TetA(C)/LuxI
- mRBS/TetA(C)/LuxI
- sRBS/TetA(C)/LuxI
- LuxI
With EcoRI-HF and SpeI (B4):
Gel electrophoresis
Gel 1
- Ps/wRBS/LuxR/attC/term/attC/mRFP
- -
- Ps/mRBS/LuxR/attC/term/attC/mRFP
- -
- Ps/sRBS/LuxR/attC/term/attC/mRFP
- -
- Pm/sRBS/LuxR/attC/term/attC/mRFP
- -
- -
- Ladder 1kb
- -
- -
File:Gel0109 1.jpg
Gel 2
- wRBS/TetA(C)/LuxI
- -
- mRBS/TetA(C)/LuxI
- -
- sRBS/TetA(C)/LuxI
- -
- LuxI
- -
- I13507/pLux/GFP
- Ladder 1 kb
- -
File:Gel0109 2.jpg
Gel extraction
- Ps/mRBS/LuxR/attC/term/attC/mRFP
- Ps/sRBS/LuxR/attC/term/attC/mRFP
- Pm/sRBS/LuxR/attC/term/attC/mRFP
- LuxI
PCR purification kit
- wRBS/LacZα
- sRBS/LacZα
- pLux
Restriction digest
5µl DNA + 10µl water + 2x1µl enzymes + 2µl buffer + 0.2µl BSA
With SpeI and PstI (B2):
- Ps/wRBS/LuxR/attC/term/attC/mRFP
- Ps/mRBS/LuxR/attC/term/attC/mRFP
- Ps/sRBS/LuxR/attC/term/attC/mRFP
- Pm/sRBS/LuxR/attC/term/attC/mRFP
With EcoRI-HF and XbaI (B4):
With XbaI and PstI and AlWNI (B2):
- wRBS/TetA(C)/LuxI
- mRBS/TetA(C)/LuxI
- sRBS/TetA(C)/LuxI
Gel electrophoresis
- -
- Ps/wRBS/LuxR/attC/term/attC/mRFP
- -
- Ps/mRBS/LuxR/attC/term/attC/mRFP
- -
- Ps/sRBS/LuxR/attC/term/attC/mRFP
- -
- Pm/sRBS/LuxR/attC/term/attC/mRFP
- -
- I13507/pLux/GFP
- -
- wRBS/TetA(C)/LuxI
- -
- mRBS/TetA(C)/LuxI
- -
- sRBS/TetA(C)/LuxI
- Ladder 1kb
File:Gel0109 3.jpg
Overnight cultures
- Pm/sRBS/LuxR/attC/term/attC/mRFP
- attP-E (pSWK)
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